FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361439
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGATCA20270.1488865825057164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1333100.0241.599733
TGACTGG1335300.0241.142295
GTGACTG1335400.0241.14014
TCGTGAC1338750.0240.920992
GACTGGG1339500.0240.260136
ACTGGGA1339950.0239.89097
CTGGGAA1364100.0235.617268
TGGGAAA1388900.0231.410139
ACGTGAC1950.0167.768942
CGAGACT3750.0151.778213
CGGGGAA3300.0142.991619
TCGAGAC3750.0142.168622
TCGTGGC4700.0141.790532
CGTGGCT4900.0138.399583
ACGGGAA1550.0132.701897
ACCGGGA3850.0125.706917
GTCGTGA2603400.0123.8648761
TGACCGG5150.0110.436925
CGTGACC5500.0105.686963
TCGCGAC1150.0105.362132
Produced by FastQC (version 0.11.5)