FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412915
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1354800.0241.433732
ACTGGGA1340550.0241.137447
GTGACTG1354000.0241.049824
TGACTGG1354800.0240.83075
TGGGAAA1329100.0239.837199
GACTGGG1358050.0239.723346
CGTGACT1380750.0236.42773
CTGGGAA1399450.0232.46778
CGTGGCT25850.0230.120963
GTGACTT6600.0187.157334
GCGTGGC5200.0184.151172
ACTGGGG13550.0175.954647
TGGGAAC27700.0165.146919
GACTGGA5650.0147.86
GTGACAG5000.0138.056064
GTGGTGA8550.0136.166871
CGTGACA4850.0134.892333
GGCGTGG7800.0127.4927751
GTCGTGA2659550.0123.1682741
TGACAGG5650.0122.134575
Produced by FastQC (version 0.11.5)