FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3163390
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT3035550.0241.54463
TGACTGG3029050.0241.507925
GTGACTG3031300.0241.43494
TCGTGAC3056600.0241.089022
ACTGGGA3015200.0241.025127
GACTGGG3037750.0240.410846
TGGGAAA3015050.0238.163359
CTGGGAA3125350.0233.526148
CGTGGCT48250.0222.898913
TCGTGGC50200.0184.897232
GTGACTT16150.0176.932684
GTCGTGG54400.0174.299121
TTGGAAA20300.0147.869989
TACCTGA4750.0145.246489
TGACTTG19700.0144.413715
GTGGTGA13900.0142.187091
ACTGGGG26100.0141.450447
CTTGGAA21050.0139.153638
ACTTGGA20900.0138.419287
CGTGACA11950.0137.83623
Produced by FastQC (version 0.11.5)