FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679487
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCAGTTTGTCGTGATCGATTTGCTTAGCT16840.10026871300581666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCA151.5912188E-5242.184283
TCGTGAC1608850.0241.569032
TGACTGG1621000.0241.411535
CGTGACT1605650.0241.332063
GTGACTG1621350.0241.231374
GACTGGG1625200.0240.862416
ACTGGGA1621450.0239.882067
CTGGGAA1650450.0235.967798
TGGGAAA1588850.0232.235069
TCGTGGC15450.0220.350372
GGTGACT28550.0169.232093
CGTGGCT22400.0168.123463
ACTTGGA8450.0167.581157
ACTGGGG15550.0166.563327
CGTGACA5600.0162.176973
GTGACTT8050.0157.89454
TGGCTGG29950.0136.597185
TTGGAAA11650.0127.7836159
TGACTTG9900.0127.151065
GTCGTGA3149600.0123.335181
Produced by FastQC (version 0.11.5)