FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218954
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTGCTCGTCGTGATGACAGTAGGCGATG200741.6468217832666368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGATG17480.14340163779765275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1196750.0241.237352
ACTGGGA1193550.0241.14997
GTGACTG1197150.0240.94764
TGACTGG1202750.0239.917865
TGGGAAA1201750.0239.333549
GACTGGG1214900.0237.319936
CGTGACT1231850.0234.140493
CTGGGAA1249650.0230.140348
CGAGACT3050.0150.829123
TCGAGAC3100.0144.574282
ACGTGAC2650.0137.128082
GACTGGA4750.0127.37436
GTCGTGA2324150.0124.201611
GCGTGGC1400.0121.129812
ACCGGGA3650.0119.338187
TGCGAAA1100.0109.991549
GACGTGA3350.0108.496741
ACTGGGG6500.0104.242417
CGGGGAA3550.095.4292769
CTGCGAA1350.089.6227348
Produced by FastQC (version 0.11.5)