FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440180
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGAATCTGGTCGTGATTACACGACCACTTG17420.12095710258439916No Hit
GTCGTGACTGGGAAAACCCTGGGGGAGAGTCGTGATGCCGCTGATACTTG15890.11033343054340429No Hit
GTCGTGACTGGGAAAACCCTGGTGGGACGTCGTGATTCTACCAAAACTTG15450.1072782568845561No Hit
GTCGTGACTGGGAAAACCCTGGTTTCCTGTCGTGATACCAAGCTGACTTG14510.10075129497701676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1382300.0241.706334
TGACTGG1382500.0241.603155
TCGTGAC1382500.0241.371282
GACTGGG1383650.0241.097296
CGTGACT1381000.0240.704243
ACTGGGA1379700.0240.525287
CTGGGAA1420800.0234.309288
TGGGAAA1374450.0231.294049
TCGTGGC15750.0221.587892
CGTGGCT18600.0210.262273
ACTGGGG10300.0168.00777
GTGGTGA19100.0161.864761
CGTGACA3950.0159.396223
GCGTGGC5350.0154.024282
GGTGACT22100.0135.872383
GTCGTGG28150.0130.930481
GTCGTGA2692500.0123.91461
CGTGACG5050.0119.881013
TGACCGG1650.0117.365885
TACCTGA3350.0115.586059
Produced by FastQC (version 0.11.5)