FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16572
Sequences flagged as poor quality0
Sequence length248
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTAGCTGGTCGTGATCTGTCACCACGATG494929.863625392227856No Hit
GTCGTGACTGGGAAAACCCTGGGTGCATGTCGTGATGCTTGGTTGCGATG10966.613565049481053No Hit
GTCGTGACTGGGAAAACCCTGGCACCTCGTCGTGATGTCCTCTCTCGATG3392.045619116582187No Hit
GTCGTGACTGGGAAAACCCTGGCTGTGAGTCGTGATAAAGAGTCTACGAT2591.5628771421675114No Hit
GTCGTGACTGGGAAAACCCTGGCCTTTCGTCGTGATATTTAGAGCCAATG2501.5085686700458605No Hit
GTCGTGACTGGGAAAACCCTGGCCGAAGGTCGTGATTAAACCGTGCAATG2161.3034033309196236No Hit
GTCGTGACTGGGAAAACCCGGGTAGCTGGTCGTGATCTGTCACCACGATG1240.7482500603427468No Hit
GTCGTGACTGGGAAAACCCGGGGTGCATGTCGTGATGCTTGGTTGCGATG1040.627564566739078No Hit
GTCGTGACTGGGAAAACCCTGGATGGGCGTCGTGATAGTTAGCCCCGATG820.4948105237750422No Hit
GTCGTGACTGGGAAAACCCTGGCATGTTGTCGTGATAGCGCCCTCCGATG680.41033067825247405No Hit
GTCGTGACTGGGAAAACCCTGGCGTAGCGTCGTGATCGTAACCCTCTATA660.39826212889210716No Hit
GTCGTGACAGGGAAAACCCTGGTAGCTGGTCGTGATCTGTCACCACGATG600.3620564808110065No Hit
GTCGTGACTGGGAAAACCCTGGGATCATGTCTAGAAGTACAGCTCCGATG550.3318851074100893No Hit
GTCGTGACTGGGAAAACCCTGGCGGACGGTCGTGATTACTACCAGCGATG550.3318851074100893No Hit
GTCGTGACTGGAAAACCCTGGGATCATGTGTAGAAGTACAGCTCCCGATG500.30171373400917206No Hit
GTGGTGACTGGGAAAACCCTGGTAGCTGGTCGTGATCTGTCACCACGATG480.2896451846488052No Hit
GTCGTGACTGGGAAAACCCTGGCACGCAGTCGTGATTCGGAACCATGGTG450.2715423606082549No Hit
GTCGTGACTGGGAAAACCCTGGAGCCACGTCGTGATACCCTACCTCGGTG420.25343953656770457No Hit
GTGGTGACTGGGAAAACCCTGGGTGCATGTCGTGATGCTTGGTTGCGATG410.2474052618875211No Hit
GTCGTGACTGGGAACACCCTGGTAGCTGGTCGTGATCTGTCACCACGATG390.23533671252715427No Hit
GTCGTGACTGGGAAAACCCTGGAAGTATGTCGTGATATGTCCTCCCGATG380.2293024378469708No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTGGTCGTGATCAGTCACCACGATG370.22326816316678735No Hit
GTCGTGACTGGGAAACCCCTGGTAGCTGGTCGTGATCTGTCACCACGATG360.2172338884866039No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTCGTCGTGATCTGTCACCACGATG350.21119961380642047No Hit
GTGGTGACTGGGAAAACCCGGGGTGCATGTCGTGATGCTTGGTTGCGATG270.16292541636495295No Hit
GTCGTGACTGGGAAAACCCTGGGATCATGTGTAGAAGTACAGCTCCGATG270.16292541636495295No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTGGTCGTGATCTGTCACCACGATG240.1448225923244026No Hit
GTCGTGACTGGGAAAACCCTGGGCCCCCGTCGTGATAGATACCCCGGAAG240.1448225923244026No Hit
GTCGTGACTGGGAAAACCCGGGCTGTGAGTCGTGATAAAGAGTCTACGAT230.13878831764421917No Hit
GTCGTGACTGGGAAAACCCTGGAGTTTCGTCGTGATTTAGGTTCTTGGTG220.13275404296403573No Hit
GTCGTGACTGGGAAAACCCTGGTGAACAGTCGTGATCAGAACTTTCGAAT200.12068549360366883No Hit
GTCGTGACTGGGAAAACCCTGGATTCCTGTCGTGATTCGTTAGCACGATG200.12068549360366883No Hit
GTCGGGACTGGGAAAACCCTGGTAGCTGGTCGTGATCTGTCACCACGATG190.1146512189234854No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTGGTCGTGATCTGTAACCACGATG180.10861694424330195No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTGGTCGTGATCTGACACCACGATG180.10861694424330195No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGG201.6120975E-7242.000026
CTGGGGA450.0242.000028
GGCGTGA201.6120975E-7242.000021
GGACTGG201.6120975E-7242.000025
TGACAGG201.6120975E-7242.000025
GTGAGTG201.6120975E-7242.000024
GCGTGGA100.0015180967242.000022
CGTGGCT950.0242.000023
CGTGGAT100.0015180967242.000023
TGGGGAG400.0242.000029
GTGGGGA100.0015180967242.000021
GGGACTG201.6120975E-7242.000024
GACTGGA201.6120975E-7242.000026
CGTGAGT201.6120975E-7242.000023
TGGGAGA201.6120975E-7242.000029
GTGGATG100.0015180967242.000024
TGAGTGG201.6120975E-7242.000025
TCGTGAC13350.0242.02
TGGGGGA500.0242.09
GTGACTG13800.0242.04
Produced by FastQC (version 0.11.5)