FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134768
Sequences flagged as poor quality0
Sequence length248
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGAAGACCGTCGTGATCTTATTAAACAGAT136871.2061496270603331No Hit
GTCGTGACTGGGAAAACCCTGGTCCCGCGTCGTGATTCGTACCCACAGTT24020.21167322307291003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG807150.0241.27975
TCGTGAC803750.0241.180592
GACTGGG818150.0240.973656
GTGACTG811150.0240.298364
ACTGGGA813300.0240.171947
ATGATCG38550.0239.236277
CGTGACT795550.0238.696013
TGGGAAA803100.0236.073189
CTGGGAA825700.0235.005338
AAGATCG50900.0233.263957
TATCGGA22150.0232.205959
TAGATCG24400.0232.141317
TTGATCG18500.0227.014797
TTATCGG11700.0225.490778
CGTGACG10500.0220.326143
GAGATCG38000.0217.85577
CACATGA13100.0216.297564
ACGAGAT10150.0215.866645
TGACGGG14750.0215.842385
TGATCGG90100.0213.833838
Produced by FastQC (version 0.11.5)