FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076468
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCAGAT15620.14510417402096484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1053750.0241.448143
TGACTGG1054750.0241.109335
GTGACTG1055250.0241.096154
TCGTGAC1057200.0240.908972
ACTGGGA1058000.0239.980657
GACTGGG1058800.0239.833056
CTGGGAA1103950.0229.926138
TGGGAAA1110950.0228.55369
ACGTGAC2000.0151.477942
ACGGGAA1650.0124.657367
GTCGTGA2049650.0124.2344741
ACCGGGA2100.0120.990967
TCGTGGC4350.0114.217852
CGAGACT3200.0109.755113
TGACCGG3400.0106.7716145
GAGATCG2050.0106.235967
CGGGGAA3350.0104.738459
GACCGGG3750.0100.0284966
GACGTGA3200.098.5156561
TCGAGAC3200.098.4606552
Produced by FastQC (version 0.11.5)