FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005493081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040547
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACAGCCGTCGTGATGAGAATGTGTAGGC23520.2260349604582974No Hit
GTCGTGACTGGGAAAACCCTGGAACAAAGTCGTGATTCTAAAGTATAGGC22940.22046096908645163No Hit
GTCGTGACTGGGAAAACCCTGGCTTTAGGTCGTGATGTCGCTCCTTTAGG18630.1790404469956667No Hit
GTCGTGACTGGGAAAACCCTGGTTGGAGTTCGTGATAAACGAAGTTTAGG17760.17067945993789804No Hit
GTCGTGACTGGGAAAACCCTGGACGAAGGTCGTGATGCCCGATTCTTAGG15390.14790297795294205No Hit
GTCGTGACTGGGAAAACCCTGGAGTGCTGTCGTGATGAAAATGGTTTAGG15370.1477107713539129No Hit
GTCGTGACTGGGAAAACCCTGGCACTGAGTCGTGATCCCTCAGGGTTAGG15270.14674973835876706No Hit
GTCGTGACTGGGAAAACCCTGGGGACAGGTCTGATACAAAGCTCATTAGG15000.14415494927187336No Hit
GTCGTGACTGGGAAAACCCTGGTGCTCCGTCGTGATCTGATTCTATTAGG13520.12993166094371517No Hit
GTCGTGACTGGGAAAACCCTGGCTGCCAGTCGTGATTGCTAGGATTTAGG13390.1286823180500256No Hit
GTCGTGACTGGGAAAACCCTGGTCGCTAGTCGTGATGGCCTGGGTTTAGG13340.1282018015524527No Hit
GTCGTGACTGGGAAAACCCTGGTAACTTGTCGTGATACTATCACCTTAGG13110.1259914256636173No Hit
GTCGTGACTGGGAAAACCCTGGACCTCAGTCGTGATACCACCACTTTAGG13070.125607012465559No Hit
GTCGTGACTGGGAAAACCCTGGCTGCGTGTCGTGATCATTGCAATTTAGG12780.12282001677963611No Hit
GTCGTGACTGGGAAAACCCTGGTTAGTTGTCGTGATGGACAGAATTTAGG12760.12262781018060694No Hit
GTCGTGACTGGGAAAACCCTGGCCTTTAGTCGTGATGACAGGACATTAGG12540.12051353759128611No Hit
GTCGTGACTGGGAAAACCCTGGCTGTAAGTCGTGATAGAGAGAAATTAGG12320.11839926500196532No Hit
GTCGTGACTGGGAAAACCCTGGCCTCTAGTCGTGATAAAAATATTTTAGG12210.11734212870730491No Hit
GTCGTGACTGGGAAAACCCTGGGCGATGGTCGTGATGATGACCAATTAGG11970.11503564951895492No Hit
GTCGTGACTGGGAAAACCCTGGGAGTGGGTCGTGATAACCGCGGTTTAGG11530.11080710434031331No Hit
GTCGTGACTGGGAAAACCCTGGGCTCATGTCGTGATGTAAGTTACTTAGG11460.11013438124371125No Hit
GTCGTGACTGGGAAAACCCTGGTAATGGGTCGTGATTTGGATTGATTAGG11350.10907724494905084No Hit
GTCGTGACTGGGAAAACCCTGGTGCTGTGTCGTGATGACATTAATTTAGG11300.10859672845147793No Hit
GTCGTGACTGGGAAAACCCTGGCTGAGTGTCGTGATAAGGCCTGCTTAGG11090.10657855916167171No Hit
GTCGTGACTGGGAAAACCCTGGCTTCAGGTCGTGATACTATCACCTTAGG11070.10638635256264253No Hit
GTCGTGACTGGGAAAACCCTGGCCGGGGGTCGTGATCAGTTGGAGTTAGG11050.10619414596361337No Hit
GTCGTGACTGGGAAAACCCTGGCCGTAGGTCGTGATTAACACACCTTAGG11020.10590583606506962No Hit
GTCGTGACTGGGAAAACCCTGGTGGCCGGTCGTGATGGTGAGCTTTTAGG11010.10580973276555503No Hit
GTCGTGACTGGGAAAACCCTGGCCAAGTGTCGTGATCAAAGTTGATTAGG10890.10465649317138005No Hit
GTCGTGACTGGGAAAACCCTGGATACTTGTCGTGATATATTTTCATTAGG10860.10436818327283631No Hit
GTCGTGACTGGGAAAACCCTGGCTAGTAGTCGTGATGTGAAAGAGTTAGG10840.10417597667380714No Hit
GTCGTGACTGGGAAAACCCTGGCGAAGTGTCGTGATTGAGGCACATTAGG10740.10321494367866131No Hit
GTCGTGACTGGGAAAACCCTGGGTCGAAGTCGTGATCCTTGGTCATTAGG10460.100524051292253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)