FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005493083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282569
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCTAGACGTCGTGATATAGAGCGTACTTG12290.434938015139665No Hit
GTCGTGACTGGGAAAACCCTGGACCTGGGTCGTGATCTCCGCCCGACTTG11890.4207821806355262No Hit
GTCGTGACTGGGAAAACCCTGGCGCCTAGTCGTGATTCCTCGTCGACTTG11080.39211661576464507No Hit
GTCGTGACTGGGAAAACCCTGGAAAGTCGTCGTGATGGGACCAAGACTTG10500.3715906557336438No Hit
GTCGTGACTGGGAAAACCCTGGTGCTTCGTCGTGATCCCTGATCCACTTG9430.3337237984350725No Hit
GTCGTGACTGGGAAAACCCTGGTAGACGGTCGTGATTAGTAACCTACTTG8280.29302577423567344No Hit
GTCGTGACTGGGAAAACCCTGGTTCTACGTCGTGATGGAGGGTCCACTTG7990.28276279422017275No Hit
GTCGTGACTGGGAAAACCCTGGAGTGCGGTCGTGATATAGCCAGTACTTG7450.26365241763958536No Hit
GTCGTGACTGGGAAAACCCTGGCTCAATGTCGTGATACAATTAAGACTTG7090.25091216658586046No Hit
GTCGTGACTGGGAAAACCCTGGTGTTATGTCGTGATATGGTCAAGACTTG6950.24595762450941183No Hit
GTCGTGACTGGGAAAACCCTGGGGACGAGTCGTGATCCCATATACACTTG6850.24241866588337715No Hit
GTCGTGACTGGGAAAACCCTGGCAACAGGTCGTGATTGGACTCCTACTTG6790.24029529070775635No Hit
GTCGTGACTGGGAAAACCCTGGGCCTTTGTCGTGATAGCTACCCTACTTG6710.23746412380692858No Hit
GTCGTGACTGGGAAAACCCTGGCTCCTGGTCGTGATGCTTACTCTACTTG6690.23675633208172162No Hit
GTCGTGACTGGGAAAACCCTGGTTAGTAGTCGTGATGAGAAGTGCACTTG6350.22472387275320363No Hit
GTCGTGACTGGGAAAACCCTGGTACCAAGTCGTGATGCGGTCTATACTTG6270.22189270585237586No Hit
GTCGTGACTGGGAAAACCCTGGGTGGAGGTCGTGATGCGTGACCCACTTG6100.2158764761881169No Hit
GTCGTGACTGGGAAAACCCTGGTTGGGGGTCGTGATCATTACGTTACTTG6000.2123375175620822No Hit
GTCGTGACTGGGAAAACCCTGCCAGGTGTCCTGATTAAAAGTTGGACTTG5950.21056803824906484No Hit
GTCGTGACTGGGAAAACCCTGGTACGGGGTCGTGATGATGAGTTTACTTG5940.21021414238646136No Hit
GTCGTGACTGGGAAAACCCTGGAAACTGGTCGTGATTGCTCGGATACTTG5840.20667518376042668No Hit
GTCGTGACTGGGAAAACCCTGGCAGACGGTCGTGATCGAAACACTACTTG5720.202428433409185No Hit
GTCGTGACTGGGAAAACCCTGGCCTATAGTCGTGATGGTAAGAGTACTTG5480.19393493270670173No Hit
GTCGTGACTGGGAAAACCCTGGCGTCCAGTCGTGATGGCGAGGAAACTTG5460.19322714098149477No Hit
GTCGTGACTGGGAAAACCCTGGTTTAATGTCGTGATAGCTTAGAGACTTG5410.19145766166847744No Hit
GTCGTGACTGGGAAAACCCTGGTTGCCGGTCGTGATGCATTGACCACTTG5320.1882725989050462No Hit
GTCGTGACTGGGAAAACCCTGGCAAGACGTCGTGTAGCGAACCCGACTTG5310.18791870304244274No Hit
GTCGTGACTGGGAAAACCCTGGCCGTAAGTCGTGATGCTGACCCCACTTG5220.1847336402790115No Hit
GTCGTGACTGGGAAAACCCTGGCCCTACGTCGTGATGCTAGGAAAACTTG5200.18402584855380455No Hit
GTCGTGACTGGGAAAACCCTGGTGCCGTGTCGTATTGAGTATCTCACTTG5200.18402584855380455No Hit
GTCGTGACTGGGAAAACCCTGGCGATTGGTCGTGATCGATTATACACTTG5150.1822563692407872No Hit
GTCGTGACTGGGAAAACCCTGGTACGCTGTCGTGATGGAGTGTTCACTTG5140.18190247337818374No Hit
GTCGTGACTGGGAAAACCCTGGACCCTGGTCGTGATACCAAAACTACTTG5000.17694793130173514No Hit
GTCGTGACTGGGAAAACCCTGGACGGCAGTCGTGATACGTTTACTACTTG4990.17659403543913169No Hit
GTCGTGACTGGGAAAACCCTGGTGACAAGTCGTGATCGGCAGTACACTTG4940.17482455612611433No Hit
GTCGTGACTGGGAAAACCCTGGGCCCGCGTCGTGATTCGTACAGCACTTG4920.1741167644009074No Hit
GTCGTGACTGGGAAAACCCTGGAACCTAGTCGTGATCGAGAGAAAACTTG4880.17270118095049353No Hit
GTCGTGACTGGGAAAACCCTGGTGTGCCGTCGTGATCGGCTTCCCACTTG4810.1702239099122692No Hit
GTCGTGACTGGGAAAACCCTGGTGCAGTGTCGTGATGGTGCTACAACTTG4750.16810053473664838No Hit
GTCGTGACTGGGAAAACCCTGGAATCAAGTCGTGATAGGTACACCACTTG4730.16739274301144144No Hit
GTCGTGACTGGGAAAACCCTGGGGCTAGGTCGTGATTGCAAGGATACTTG4640.16420768024801022No Hit
GTCGTGACTGGGAAAACCCTGGCATGGCGTCGTGATAGAAAGTAAACTTG4610.1631459926601998No Hit
GTCGTGACTGGGAAAACCCTGGATTTAAGTCGTGATGAGAGGTACACTTG4600.16279209679759635No Hit
GTCGTGACTGGGAAAACCCTGCCAGGTGTCGTGATTAAAAGTTGGACTTG4540.1606687216219755No Hit
GTCGTGACTGGGAAAACCCTGGTTACGTGTCGTGATATTCTGTCTACTTG4440.1571297629959408No Hit
GTCGTGACTGGGAAAACCCTGGCCCCGTGTCGTGATATGCGCGGCACTTG4380.15500638782032No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGGGTCGTGATGCAAAGTTGACTTG4370.15465249195771652No Hit
GTCGTGACTGGGAAAACCCTGGTCCATCGTCGTGATCCCGGGTCTACTTG4370.15465249195771652No Hit
GTCGTGACTGGGAAAACCCTGGTAAATAGTCGTGATGGGCGAGGGACTTG4290.15182132505688875No Hit
GTCGTGACTGGGAAAACCCTGGGAGTTGGTCGTGATCAAAATCACACTTG4230.14969794988126794No Hit
GTCGTGACTGGGAAAACCCTGGTTCATAGCTTGCGATGCTCTTCAACTTG4200.14863626229345753No Hit
GTCGTGACTGGGAAAACCCTGGGATGCCGTCGTGATCCACACGAGACTTG4090.14474340780481937No Hit
GTCGTGACTGGGAAAACCCTGGAACGCTGTCGTGATGCCTAGAGTACTTG4080.1443895119422159No Hit
GTCGTGACTGGGAAAACCCTGGTAATAAGTCGTGATCTCTGCGCGACTTG4060.14368172021700895No Hit
GTCGTGACTGGGAAAACCCTGGCATTGTGTCGTGATTAACGTGTAACTTG4060.14368172021700895No Hit
GTCGTGACTGGGAAAACCCTGGCGTTGTGTCGTGATTCATGATGGACTTG4050.14332782435440547No Hit
GTCGTGACTGGGAAAACCCTGGTAGTTAGTCGTGATGCATGTGGAACTTG4040.142973928491802No Hit
GTCGTGACTGGGAAAACCCTGGATAGACGTCGTGATGGCGATTGAACTTG4020.14226613676659505No Hit
GTCGTGACTGGGAAAACCCTGGTATCCTGTCGTGATCATAGCCATACTTG3990.14120444917878464No Hit
GTCGTGACTGGGAAAACCCTGGGACCTTGTCGTGATTTGATCTGTACTTG3980.14085055331618118No Hit
GTCGTGACTGGGAAAACCCTGGGCAGAAGTCGTGATACGTAATACACTTG3970.1404966574535777No Hit
GTCGTGACTGGGAAAACCCTGGCAAATTGTCGTGATCGTGTCACAACTTG3890.13766549055274996No Hit
GTCGTGACTGGGAAAACCCTGGGATCGGGTCGTGATCGAGAGGGGACTTG3870.136957698827543No Hit
GTCGTGACTGGGAAAACCCTGGATTTCAGTCGTGATCAATAGTATACTTG3860.13660380296493954No Hit
GTCGTGACTGGGAAAACCCTGGAATAGCGTCGTGATTCTAGGTAGACTTG3830.13554211537712912No Hit
GTCGTGACTGGGAAAACCCTGGAAAGCTGTCGTGATTTTATTACCACTTG3810.1348343236519222No Hit
GTCGTGACTGGGAAAACCCTGGCAAGTGGTCGTGATCTGGCGAGCACTTG3790.13412653192671525No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGAGTCGTGATAAAAACACCACTTG3700.130941469163284No Hit
GTCGTGACTGGGAAAACCCTGGCCTACCGTCGTGATTTCGTGGCCACTTG3690.13058757330068055No Hit
GTCGTGACTGGGAAAACCCTGGCCTGTCGTCGTGATCGGAGTAGAACTTG3650.12917198985026665No Hit
GTCGTGACTGGGAAAACCCTGGTTACCCGTCGTGATTCGAATAGTACTTG3600.1274025105372493No Hit
GTCGTGACTGGGAAAACCCTGGATTTGGGTCGTGATACGGATTACACTTG3590.12704861467464584No Hit
GTCGTGACTGGGAAAACCCTGGTGAACGGTCGTGATCCCTCACTCACTTG3570.12634082294943888No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTGGTCGTGATCTGTCACCAACTTG3560.12598692708683543No Hit
GTCGTGACTGGGAAAACCCTGGTGACCAGTCGTGATCGAATTTCTACTTG3550.12563303122423197No Hit
GTCGTGACTGGGAAAACCCTGGCGCGTAGTCGTGATCCAACATTCACTTG3550.12563303122423197No Hit
GTCGTGACTGGGAAAACCCTGGCTAGAGGTCGTGATCGCCAGGGGACTTG3540.1252791353616285No Hit
GTCGTGACTGGGAAAACCCTGGGTTAGGGTCGTGATGTACTGGGCACTTG3530.12492523949902501No Hit
GTCGTGACTGGGAAAACCCTGGAATACGGTCGTGATCGTAGCTCCACTTG3510.12421744777381807No Hit
GTCGTGACTGGGAAAACCCTGGGGGAGTGTCGTGATACCGGCTTCACTTG3510.12421744777381807No Hit
GTCGTGACTGGGAAAACCCTGGTAAACAGTCGTGATGCAAGGGATACTTG3490.12350965604861112No Hit
GTCGTGACTGGGAAAACCCTGGTCGCGCGTCGTGATCTAACACGAACTTG3470.1228018643234042No Hit
GTCGTGACTGGGAAAACCCTGGGGATAAGTCGTGATAAGACGTACACTTG3450.12209407259819724No Hit
GTCGTGACTGGGAAAACCCTGGTCTGATGTCGTGATCTGAACACAACTTG3440.12174017673559379No Hit
GTCGTGACTGGGAAAACCCTGGCAAACCGTCGTGATCCGACCAGTACTTG3440.12174017673559379No Hit
GTCGTGACTGGGAAAACCCTGGCCTATCGTCGTGATCATCTGACTACTTG3420.12103238501038685No Hit
GTCGTGACTGGGAAAACCCTGGATATTCGTCGTGATGCCCTGGAGACTTG3390.11997069742257643No Hit
GTCGTGACTGGGAAAACCCTGGATTAGAGTCGTGATGTACATGATACTTG3370.11926290569736948No Hit
GTCGTGACTGGGAAAACCCTGGATAATAGTCGTGATTTAAATACCACTTG3350.11855511397216255No Hit
GTCGTGACTGGGAAAACCCTGGCTTCGTGTCGTGATTTACCGTAGACTTG3350.11855511397216255No Hit
GTCGTGACTGGGAAAACCCCTGGCCCCCGTCGTGATGGGACGCCCACTTG3340.11820121810955908No Hit
GTCGTGACTGGGAAAACCCTGGACAAGAGTCGTGATCTGTACGGGACTTG3330.11784732224695563No Hit
GTCGTGACTGGGAAAACCCTGGGTGGCGGTCATTCACAGTACCTGACTTG3310.11713953052174866No Hit
GTCGTGACTGGGAAAACCCTGGCAAATCGTCGTGATCTCTCTGGAACTTG3290.11643173879654174No Hit
GTCGTGACTGGGAAAACCCTGGACAAACGTCGTGATTTAAACATAACTTG3270.11572394707133479No Hit
GTCGTGACTGGGAAAACCCTGGAAGACCGTCGTGATGGAATGTTCACTTG3220.11395446775831744No Hit
GTCGTGACTGGGAAAACCCTGGCGTACTGTCGTGATCCAAATTACACTTG3190.11289278017050702No Hit
GTCGTGACTGGGAAAACCCTGGATCGGCGTCTGATATCGTACCAGACTTG3180.11253888430790356No Hit
GTCGTGACTGGGAAAACCCTGGGCCCGCTGTCGTGTCTGAGTCTGACTTG3180.11253888430790356No Hit
GTCGTGACTGGGAAAACCCTGGCATCAGGTCGTGATGTATTGCTTACTTG3160.1118310925826966No Hit
GTCGTGACTGGGAAAACCCTGGGCCCTGGTCGTGATACTAATGCCACTTG3140.11112330085748967No Hit
GTCGTGACTGGGAAAACCCTGGTAATTGGTCGTGATTGTATTGATACTTG3130.11076940499488622No Hit
GTCGTGACTGGGAAAACCCTGGTTTAAGGTCGTGTAGATGGGAGGACTTG3130.11076940499488622No Hit
GTCGTGACTGGGAAAACCCTGGATCCGTGTCGTGATTAAAATAGGACTTG3130.11076940499488622No Hit
GTCGTGACTGGGAAAACCCTGGCCGAAAGTCGTGATAATTAGGAGACTTG3120.11041550913228275No Hit
GTCGTGACTGGGAAAACCCTGGTGACACGTCGTGATTCTGTCTCCACTTG3120.11041550913228275No Hit
GTCGTGACTGGGAAAACCCTGGGGAGCTGTCGTGATGTTCTGGTAACTTG3110.11006161326967925No Hit
GTCGTGACTGGGAAAACCCTGGGTCCGTGTCGTGATATGAACACTACTTG3080.10899992568186886No Hit
GTCGTGACTGGGAAAACCCTGGCTACAAGTCGTGATAGGAGCAGAACTTG3070.10864602981926538No Hit
GTCGTGACTGGGAAAACCCTGGTGATGTGTCGTGATCCCGCGGACACTTG3060.10829213395666192No Hit
GTCGTGACTGGGAAAACCCTGGTGGAAAGTCGTGATTGGTGAGGAACTTG3060.10829213395666192No Hit
GTCGTGACTGGGAAAACCCTGGTTACGCGTCGTGATGCTCCCCCTACTTG3050.10793823809405845No Hit
GTCGTGACTGGGAAAACCCTGGGCCAACGTCGTGATTCCGGCTACACTTG3040.10758434223145498No Hit
GTCGTGACTGGGAAAACCCTGGCTCACCGTCGTGATTTGAATGGGACTTG3040.10758434223145498No Hit
GTCGTGACTGGGAAAACCCTGGTATCCTGTCGTGATTCAGAGATCACTTG2980.10546096705583415No Hit
GTCGTGACTGGGAAAACCCTGGCTTTTAGTCGTGATCGTTACTCGACTTG2980.10546096705583415No Hit
GTCGTGACTGGGAAAACCCTGGTGGTTCGTCGTGATTGGAAGATAACTTG2950.10439927946802373No Hit
GTCGTGACTGGGAAAACCCTGGTGAGTCGTCGTGATCATGCACATACTTG2940.10404538360542026No Hit
GTCGTGACTGGGAAAACCCTGGCGGTCTGTCGTGATCTCCCTTGTACTTG2920.10333759188021334No Hit
GTCGTGACTGGGAAAACCCTGGTCTCCTGTCGTGATACGACCGTGACTTG2900.10262980015500638No Hit
GTCGTGACTGGGAAAACCCTGGCTGCTGGTCGTGATCATAACAAGACTTG2870.10156811256719599No Hit
GTCGTGACTGGGAAAACCCTGGCAGGAGGTCGTGATTCAGTAAGCACTTG2860.1012142167045925No Hit
GTCGTGACTGGGAAAACCCTGGAAGCGTGTCGTGATGTACCGCACACTTG2850.10086032084198904No Hit
GTCGTGACTGGGAAAACCCTGGGTTGCCGTCGTGATCTTATGCGGACTTG2830.1001525291167821No Hit
GTCGTGACTGGGAAAACCCTGGGCTCGGGTCGTGATCCGTGTTGAACTTG2830.1001525291167821No Hit
GTCGTGACTGGGAAAACCCTGGTCACACGTCGTGATCCGGAGGGCACTTG2830.1001525291167821No Hit
GTCGTGACTGGGAAAACCCTGGCCGGATGTCGTGATCTAGTCTGAACTTG2830.1001525291167821No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)