FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735342
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT4662250.0241.44353
TCGTGAC4671500.0241.169862
TGACTGG4670200.0241.024735
GTGACTG4670100.0241.012884
ACTGGGA4666600.0240.658747
GACTGGG4678200.0240.343616
CTGGGAA4826450.0232.630518
TGGGAAA4902400.0229.02419
ACGTGAC7150.0166.069992
GTCGTGA9093900.0123.819321
ACCGGGA11450.0123.637557
CGAGACT12200.0112.1455843
CGTGGCT15750.0103.0123
CGGGGAA12400.099.5287259
ACTGGAA25950.091.8541647
GACGTGA12150.090.777591
ACTGGGG23650.087.4852757
TCGAGAC13650.086.989052
ACGGGAA5700.082.7865457
TCGTGGC20100.080.1727452
Produced by FastQC (version 0.11.5)