FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005493085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618151
Sequences flagged as poor quality0
Sequence length248
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCACAATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT30200.1866327678937256Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT20340.1256990231443172Illumina Single End PCR Primer 1 (97% over 38bp)
GACCACAATATCGGAACAGCGTCGTGTAGGGAAAGAGTGTAGATCCAGAT20050.12390685418109929Illumina Single End PCR Primer 1 (96% over 28bp)
GACCACAATATCGGAACACCGTCGTGTAGGCAAAGAGTGTAGATCCAGAT17740.10963130140512227No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)