FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005493090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452002
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCAGTGCGGTCGTGATTCTGTAACTGACC16020.3544232105167676No Hit
GTCGTGACTGGGAAAACCCTGGCGGTCAGTCGTGTTAAAATAGGATGACC12320.27256516564086003No Hit
GTCGTGACTGGGAAAACCCTGGATCTACGTCGTGATCCGCGGTTCTGACC12090.26747669258100626No Hit
GTCGTGACTGGGAAAACCCTGGCTGAGGGTCGTGATACTAAGACTTGACC11780.26061831584816No Hit
GTCGTGACTGGGAAAACCCTGGTCAGAGGTCGTGAATATATGTACTGACC11680.2584059362569192No Hit
GTCGTGACTGGGAAAACCCTGGCGAAACGTCGTGATGGTGTGACCTGACC11420.25265374931969325No Hit
GTCGTGACTGGGAAAACCCTGGAAGCTTGTCGTATACGTTGGAAATGACC10680.23628214034451173No Hit
GTCGTGACTGGGAAAACCCTGGATGACCGTCGTGATCGTAAGACTTGACC10340.2287600497342932No Hit
GTCGTGACTGGGAAAACCCTGGCCCACCGTCGTGATCAATTCATTTGACC10110.2236715766744395No Hit
GTCGTGACTGGGAAAACCCTGGGCCAATGTCGTGATTATAAAGTTTGACC10090.22322910075619132No Hit
GTCGTGACTGGGAAAACCCTGGCCACTAGTCGTGATGTGCCCAGATGACC9970.22057424524670244No Hit
GTCGTGACTGGGAAAACCCTGGACCAGTGTCGTGATGCGTGCCCTTGACC9870.2183618656554617No Hit
GTCGTGACTGGGAAAACCCTGGAATTTGGTCGTGATAAACATGGCTGACC9650.21349463055473206No Hit
GTCGTGACTGGGAAAACCCTGGCGGCACGTCGTGATTGAATGATTTGACC9630.21305215463648391No Hit
GTCGTGACTGGGAAAACCCTGGTGGGAAGTCGTGATACGAAGTGGTGACC9520.21061853708611908No Hit
GTCGTGACTGGGAAAACCCTGGACTGTGGTCGTGATACAGAAGATTGACC9390.20774244361750613No Hit
GTCGTGACTGGGAAAACCCTGGTGTCTAGTCGTGATCCGAATGTGTGACC9390.20774244361750613No Hit
GTCGTGACTGGGAAAACCCTGGGACGCCGTCGTGATGCATGTTGCTGACC9300.20575130198538946No Hit
GTCGTGACTGGGAAAACCCTGGCAATAAGTCGTGATCTATACGGATGACC9170.20287520851677648No Hit
GTCGTGACTGGGAAAACCCTGGGCATAGGTCGTGATTGGAGCCGTTGACC9050.2002203530072876No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGAGTCGTGATAATGATAGCTGACC9050.2002203530072876No Hit
GTCGTGACTGGGAAAACCCTGGTTCTTCGTCGTGATCAAAAGTCCTGACC8720.19291950035619312No Hit
GTCGTGACTGGGAAAACCCTGGGAGCAAGTCGTGATAATCAGTTGTGACC8690.19225578647882088No Hit
GTCGTGACTGGGAAAACCCTGGCAATACGTCGTGATAGCCCCTCTTGACC8660.19159207260144867No Hit
GTCGTGACTGGGAAAACCCTGGTAAAAAGTCGTGATCAACTGAGCTGACC8640.1911495966832005No Hit
GTCGTGACTGGGAAAACCCTGGAAGCGCGTCGTGATAACATTTGATGACC8490.18783102729633938No Hit
GTCGTGACTGGGAAAACCCTGGTGGTTTGTCGTGATGGCATAGCGTGACC8480.1876097893372153No Hit
GTCGTGACTGGGAAAACCCTGGTTGTACGTCGTGATACGGGGCTGTGACC8430.18650359954159496No Hit
GTCGTGACTGGGAAAACCCTGGCTAGCCGTCGTGATGCAAAGTGGTGACC8340.1845124579094783No Hit
GTCGTGACTGGGAAAACCCTGGATAACTGCGTATGAGGCTTTGGTTGACC8230.18207884035911345No Hit
GTCGTGACTGGGAAAACCCTGGCTCACCGTCGTGATCCTGAGGATTGACC8110.17942398484962457No Hit
GTCGTGACTGGGAAAACCCTGGTACCGGGTCGTGATCCGTGCCCGTGACC8110.17942398484962457No Hit
GTCGTGACTGGGAAAACCCTGGCCATCTGTCGTGATTGGTACTTGTGACC8090.1789815089313764No Hit
GTCGTGACTGGGAAAACCCTGGATTCGTGTCGTGATGCATGATCATGACC8080.17876027097225233No Hit
GTCGTGACTGGGAAAACCCTGGCGCTAAGTCGTGATCTTGCTGGGTGACC8050.1780965570948801No Hit
GTCGTGACTGGGAAAACCCTGGGCCTGGGTCGTGATTGCATAGGGTGACC8000.17699036729925974No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTCGTCGTGATGTAGGTAGCTGACC7960.17610541546276345No Hit
GTCGTGACTGGGAAAACCCTGGCGCTTCGTCGTGATGCGTACCGTTGACC7900.17477798770801897No Hit
GTCGTGACTGGGAAAACCCTGGAGACCGGTCGTGATCCTATACCGTGACC7840.17345055995327455No Hit
GTCGTGACTGGGAAAACCCTGGGCGATTGTCGTGATTCAGAGTTTTGACC7740.1712381803620338No Hit
GTCGTGACTGGGAAAACCCTGGCGCATGGTCGTGATGTACAAAGCTGACC7660.1694682766890412No Hit
GTCGTGACTGGGAAAACCCTGGTTAGAGGTCGTGATAGCGCGAGCTGACC7620.1685833248525449No Hit
GTCGTGACTGGGAAAACCCTGGTGACCTGTCGTGATGAGCGGGAGTGACC7560.16725589709780045No Hit
GTCGTGACTGGGAAAACCCTGGGGCATCGTCGTGATACCTGCTCCTGACC7550.16703465913867638No Hit
GTCGTGACTGGGAAAACCCTGGCCGTAAGTCGTGATATTTGCGGATGACC7540.1668134211795523No Hit
GTCGTGACTGGGAAAACCCTGGACTTTGGTCGTGATGCCCGGTCCTGACC7400.16371608975181526No Hit
GTCGTGACTGGGAAAACCCTGGATTTCGGTCGTGATTGTATCCCGTGACC7380.16327361383356712No Hit
GTCGTGACTGGGAAAACCCTGGAACCAGGTCGTATCCCCCAAGGATGACC7370.16305237587444305No Hit
GTCGTGACTGGGAAAACCCTGGACGCGGGTCGTGATCGTAAGACCTGACC7370.16305237587444305No Hit
GTCGTGACTGGGAAAACCCTGGACGGACGTCGTGATCGAATGGAATGACC7290.16128247220145042No Hit
GTCGTGACTGGGAAAACCCTGGCAACGTGTCGTGATCTGAGGATATGACC7250.16039752036495414No Hit
GTCGTGACTGGGAAAACCCTGGCGTCGGGTCGTGATGAGGTAGGGTGACC7250.16039752036495414No Hit
GTCGTGACTGGGAAAACCCTGGCCTAAAGTCGTGATGGTCTGCTATGACC7210.15951256852845785No Hit
GTCGTGACTGGGAAAACCCTGGTCAATTGTCGTGATTCTAACGCCTGACC7210.15951256852845785No Hit
GTCGTGACTGGGAAAACCCTGGAATGTGGTCGTGATCCCGAGTTGTGACC7190.15907009261020968No Hit
GTCGTGACTGGGAAAACCCTGGACCAGGGTCGTGATGGGCACACTTGACC7090.15685771301896895No Hit
GTCGTGACTGGGAAAACCCTGGAGATCGGTCGTGATAACAAGGAATGACC7030.1555302852642245No Hit
GTCGTGACTGGGAAAACCCTGGGCACCGGTCGTGATTGAAAACCCTGACC7010.15508780934597635No Hit
GTCGTGACTGGGAAAACCCTGGGCAACCGTCGTGATCTGTACGAGTGACC7000.15486657138685228No Hit
GTCGTGACTGGGAAAACCCTGGGTACGGGTCGTGATTATGATGTATGACC7000.15486657138685228No Hit
GTCGTGACTGGGAAAACCCTGGACCTAAGTCGTGATCTAGAGAGCTGACC6950.1537603815912319No Hit
GTCGTGACTGGGAAAACCCTGGTTTTACGTCGTGATCTAAACTGATGACC6940.15353914363210783No Hit
GTCGTGACTGGGAAAACCCTGGCATGCGGTCGTGATTCAGATGAGTGACC6840.15132676404086706No Hit
GTCGTGACTGGGAAAACCCTGGCTGACAGTCGTGATACTGATCCCTGACC6750.14933562240875042No Hit
GTCGTGACTGGGAAAACCCTGGCGAGCGGTCGTGATTATGATAGTTGACC6720.14867190853137818No Hit
GTCGTGACTGGGAAAACCCTGGTTGTCAGTCGTGATAGTAAGGAATGACC6700.14822943261313No Hit
GTCGTGACTGGGAAAACCCTGGAATGTCGTCGTGATTCGAAGAGGTGACC6690.14800819465400594No Hit
GTCGTGACTGGGAAAACCCTGGCTACAAGTCGTGATGCGGAGGGATGACC6660.14734448077663373No Hit
GTCGTGACTGGGAAAACCCTGGGCACGGGTCGTGATGTAGGTAGGTGACC6590.1457958150627652No Hit
GTCGTGACTGGGAAAACCCTGGGAACTGGTCGTGATAATGACTGTTGACC6550.14491086322626892No Hit
GTCGTGACTGGGAAAACCCTGGACCCGAGTCGTGATCCAAAGACGTGACC6550.14491086322626892No Hit
GTCGTGACTGGGAAAACCCTGGGCCGCAGTCGTGATGCTGATCCGTGACC6530.14446838730802075No Hit
GTCGTGACTGGGAAAACCCTGGGAGGACGTCGTGATAGTCTTTGATGACC6510.1440259113897726No Hit
GTCGTGACTGGGAAAACCCTGGCAATTCGTCGTGATACATTGATCTGACC6480.1433621975124004No Hit
GTCGTGACTGGGAAAACCCTGGTCTAGGGTCGTGATGTGAAGTATTGACC6470.1431409595532763No Hit
GTCGTGACTGGGAAAACCCTGGGTAGATGTCGTGATGGTACTAAGTGACC6450.14269848363502816No Hit
GTCGTGACTGGGAAAACCCTGGCTTCGTGTCGTGATCCAAAGTCGTGACC6440.14247724567590409No Hit
GTCGTGACTGGGAAAACCCTGGTATGTCGTCGTGATTATCAGTTATGACC6290.13915867628904297No Hit
GTCGTGACTGGGAAAACCCTGGAGCTAGGTCGTGATGGGGATCCATGACC6270.13871620037079482No Hit
GTCGTGACTGGGAAAACCCTGGTATTTAGTCGTGATCTCCAAGGATGACC6220.13761001057517444No Hit
GTCGTGACTGGGAAAACCCTGGAGCCCTGTCGTGATATATATAGCTGACC6120.1353976309839337No Hit
GTCGTGACTGGGAAAACCCTGGGTCGTAGTCGTGATCGTAAGGATTGACC6060.13407020322918925No Hit
GTCGTGACTGGGAAAACCCTGGTCCATAGTCGTGATTCCTCGCCCTGACC6060.13407020322918925No Hit
GTCGTGACTGGGAAAACCCTGGGGTGATGTCGTGATTAGGCAAATTGACC5990.13252153751532073No Hit
GTCGTGACTGGGAAAACCCTGGTTTTCCGTCGTGATGCCGTGATTTGACC5950.1316365856788244No Hit
GTCGTGACTGGGAAAACCCTGGCCAGTGGTCGTGATTAGAAGGTGTGACC5950.1316365856788244No Hit
GTCGTGACTGGGAAAACCCTGGATCTGTGTCGTGATTCCTAGAGGTGACC5930.13119410976057627No Hit
GTCGTGACTGGGAAAACCCTGGCCAACGGTCGTGATTGCTGTCACTGACC5910.13075163384232813No Hit
GTCGTGACTGGGAAAACCCTGGGCGTACGTCGTGATTGAAACCCTTGACC5880.13008791996495592No Hit
GTCGTGACTGGGAAAACCCTGGTGCCCGGTCGTGATTGGCATGAGTGACC5870.12986668200583182No Hit
GTCGTGACTGGGAAAACCCTGGCAGTAGGTCGTGAACAAAGATTTTGACC5850.12942420608758368No Hit
GTCGTGACTGGGAAAACCCTGGACATAGGTCGTGATTTACTAGCCTGACC5770.1276543024145911No Hit
GTCGTGACTGGGAAAACCCTGGCGCTTCGTCGTGATTATAATGAGTGACC5770.1276543024145911No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTCGTCGTGATATCTACGCCTGACC5770.1276543024145911No Hit
GTCGTGACTGGGAAAACCCTGGAATGATGTCGTGATTGCGCGGGTTGACC5740.12699058853721884No Hit
GTCGTGACTGGGAAAACCCTGGAGACGGGTCGTGATCATTACCGGTGACC5730.12676935057809477No Hit
GTCGTGACTGGGAAAACCCTGGTCATCCGTCGTGATTAACTCACCTGACC5680.12566316078247441No Hit
GTCGTGACTGGGAAAACCCTGGAATCTTGTCGTGATGAACATTGATGACC5660.12522068486422627No Hit
GTCGTGACTGGGAAAACCCTGGAAAACGGTCGTGATAATAGGTGCTGACC5610.12411449506860589No Hit
GTCGTGACTGGGAAAACCCTGGTATCTCGTCGTGATAATGTAATCTGACC5590.12367201915035775No Hit
GTCGTGACTGGGAAAACCCTGGTGACGAGTCGTGATCCCTGTTCCTGACC5540.12256582935473737No Hit
GTCGTGACTGGGAAAACCCTGGATAAAGGTCGTGATAGCGTGAGCTGACC5540.12256582935473737No Hit
GTCGTGACTGGGAAAACCCTGGGAGGAGGTCGTGATCTGCTCGTTTGACC5520.12212335343648921No Hit
GTCGTGACTGGGAAAACCCTGGTACAACGTCGTGATCGATCAGCCTGACC5520.12212335343648921No Hit
GTCGTGACTGGGAAAACCCTGGGTCCTGGTCGTGATGTACGTTTTTGACC5500.12168087751824107No Hit
GTCGTGACTGGGAAAACCCTGGTCGCCCGTCGTGATTTAGTGTATTGACC5490.121459639559117No Hit
GTCGTGACTGGGAAAACCCTGGTGCAGCGTCGTGATTCGGATCAATGACC5480.12123840159999291No Hit
GTCGTGACTGGGAAAACCCTGGCCAGATGTCGTGATCCCCAAGCCTGACC5470.12101716364086884No Hit
GTCGTGACTGGGAAAACCCTGGGGCATTGTCGTGATTCAGGACCATGACC5400.11946849792700033No Hit
GTCGTGACTGGGAAAACCCTGGCGCTTTGTCGTGATAGAAACCCGTGACC5390.11924725996787626No Hit
GTCGTGACTGGGAAAACCCTGGCTATACGTCGTGATTGAATTGCTTGACC5360.11858354609050402No Hit
GTCGTGACTGGGAAAACCCTGGCGAGTGGTCGTGTGAGTGTCCCGTGACC5360.11858354609050402No Hit
GTCGTGACTGGGAAAACCCTGGATCATTGTCGTGATGTGAAGAGATGACC5350.11836230813137993No Hit
GTCGTGACTGGGAAAACCCTGGTGGAAGGTCGTGATCCTGTTCTTTGACC5340.11814107017225586No Hit
GTCGTGACTGGGAAAACCCTGGTAATTGGTCGTGATCCATGTTCTTGACC5330.11791983221313179No Hit
GTCGTGACTGGGAAAACCCTGGTCCTACGTCGTGATGGCGCTGGGTGACC5300.11725611833575958No Hit
GTCGTGACTGGGAAAACCCTGGGACTAGGTCGTGATTGGTATTGCTGACC5300.11725611833575958No Hit
GTCGTGACTGGGAAAACCCTGGATTCCCGTCGTGATTGTAAAGCCTGACC5300.11725611833575958No Hit
GTCGTGACTGGGAAAACCCTGGATCGGTGTCGTGATGAAATTTACTGACC5250.11614992854013921No Hit
GTCGTGACTGGGAAAACCCTGGTGAAGAGTCGTGATAGAACAAAATGACC5250.11614992854013921No Hit
GTCGTGACTGGGAAAACCCTGGAAGTCCGTCGTACGGTAGTAGCCTGACC5250.11614992854013921No Hit
GTCGTGACTGGGAAAACCCTGGTGGCTTGTCGTGATGTCTAGCCGTGACC5170.1143800248671466No Hit
GTCGTGACTGGGAAAACCCTGGCAAAGTGTCGTGATTCATATGGGTGACC5170.1143800248671466No Hit
GTCGTGACTGGGAAAACCCTGGCCTTGTGTCGTGATTGATACCGGTGACC5130.1134950730306503No Hit
GTCGTGACTGGGAAAACCCTGGACTTGCGTCGTGATAGAAACGGCTGACC5130.1134950730306503No Hit
GTCGTGACTGGGAAAACCCTGGCAATCGGTCGTGATATAAACAGCTGACC5120.11327383507152623No Hit
GTCGTGACTGGGAAAACCCTGGCTTATCGTCGTGATACGGAGCAGTGACC5120.11327383507152623No Hit
GTCGTGACTGGGAAAACCCTGGGTGACCGTCGTGATGAAAGTTGATGACC5100.11283135915327808No Hit
GTCGTGACTGGGAAAACCCTGGGGCTGAGTCGTGATCTGTACCCGTGACC5050.11172516935765771No Hit
GTCGTGACTGGGAAAACCCTGGTCCAATGTCGTGATAAGTCCTGCTGACC5020.1110614554802855No Hit
GTCGTGACTGGGAAAACCCTGGTTATAGGTCGTGATCCGTACAACTGACC5000.11061897956203734No Hit
GTCGTGACTGGGAAAACCCTGGAGCGCCGTCGTGATTAAGGATGATGACC4950.10951278976641696No Hit
GTCGTGACTGGGAAAACCCTGGAACGGTGTCGTGATGCGCACCACTGACC4920.10884907588904473No Hit
GTCGTGACTGGGAAAACCCTGGACAAGGGTCGTGATGACTATGAGTGACC4910.10862783792992066No Hit
GTCGTGACTGGGAAAACCCTGGAACGTTGTCGTGATCGCAACCCGTGACC4880.10796412405254845No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGTGTCGTGATTCAGCGCCGTGACC4860.10752164813430029No Hit
GTCGTGACTGGGAAAACCCTGGATTATGGTCGTGATCTGTACCCGTGACC4850.10730041017517622No Hit
GTCGTGACTGGGAAAACCCTGGTTGAGCGTCGTGATGTATGTAACTGACC4850.10730041017517622No Hit
GTCGTGACTGGGAAAACCCTGGTTCTGGGTCGTGATATCAAGTCCTGACC4840.10707917221605215No Hit
GTCGTGACTGGGAAAACCCTGGTTACCAGTCGTGATAACAGCCCCTGACC4830.10685793425692808No Hit
GTCGTGACTGGGAAAACCCTGGGTCAATGTCGTGATAATAACACCTGACC4780.10575174446130768No Hit
GTCGTGACTGGGAAAACCCTGGCCTGGTGTCGTGATTAGCAATTCTGACC4780.10575174446130768No Hit
GTCGTGACTGGGAAAACCCTGGCAATCAGTCGTGATGGTGTACCCTGGTC4740.10486679262481138No Hit
GTCGTGACTGGGAAAACCCTGGGTCAGCGTCGTGATCTGCACGCGTGACC4740.10486679262481138No Hit
GTCGTGACTGGGAAAACCCTGGATGGCGGTCGTGATTCCCATATATGACC4720.10442431670656324No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGCGTCGTGATGCTTTGTTGTGACC4710.10420307874743917No Hit
GTCGTGACTGGGAAAACCCTGGCACCCAGTCGTGATAATGACCCGTGACC4710.10420307874743917No Hit
GTCGTGACTGGGAAAACCCTGGAGCCGCGTCGTGATGGGGGTGATTGACC4690.10376060282919101No Hit
GTCGTGACTGGGAAAACCCTGGCACGTTGTCGTGATCTTCAGGAATGACC4680.10353936487006694No Hit
GTCGTGACTGGGAAAACCCTGGACCGTAGTCGTGATGAAATCCCGTGACC4670.10331812691094287No Hit
GTCGTGACTGGGAAAACCCTGGCCAATGGTCGTGATAGAATCTCCTGACC4650.10287565099269473No Hit
GTCGTGACTGGGAAAACCCTGGTGCAAGGTCGTGATCTATAGTTATGACC4650.10287565099269473No Hit
GTCGTGACTGGGAAAACCCTGGACAAGAGTCGTGATGAGGACACTTGACC4620.1022119371153225No Hit
GTCGTGACTGGGAAAACCCTGGTGGTAAGTCGTGATCTGTCTGAATGACC4610.10199069915619843No Hit
GTCGTGACTGGGAAAACCCTGGTGACTGGTCGTGATCCCTGATGGTGACC4590.10154822323795029No Hit
GTCGTGACTGGGAAAACCCTGGCGATATGTCGTGATGTTCCGTACTGACC4590.10154822323795029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)