FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38675856
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCA468050.12101865308423944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTGG144800.018.1994531
ATGGGAG109600.017.7482411
GGGACCT93750.017.687221
TGAGCCG114750.017.5617851
GGGAATG79600.017.4641721
CGAGCAC116250.017.4298151
TGCGGGT100500.017.4147911
TGGCCAG123600.017.3400361
CCGGGAT90550.017.2630231
TATGCCC155850.017.2491931
GACTGCG112100.017.1918431
CGGACTG68450.017.1822551
GACCTGG86800.017.1101191
CGCTGGA113650.017.0717721
ACGAGGA100450.017.0650331
TGACGGC68050.017.0187131
GCGCGAT56950.016.9991781
TTGACTT218450.016.9759461
ATGCGAT83000.016.9656641
ACGAGCC92650.016.9580961
Produced by FastQC (version 0.11.5)