FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17186090
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC245610314.291226218412682No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC11523016.7048467685203565No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC7089734.12527224051544No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3417801.9887013276434606No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT1021580.5944225824489456No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT933190.542991454135292No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA748140.4353171663828131No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA707600.4117283221489006No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC679920.3956222735945174No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT454190.26427768037988864No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT440410.25625956805765593No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA434480.25280910317588234No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT419940.2443487727575033No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG373280.2171989091177807No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG373110.21709999191206375No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA330550.1923357785278676No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC324510.188821308395336No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC310700.18078574009562384No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG298480.17367533860232315No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAG285400.16606453242127792No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG278210.16188091648536695No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC251820.14652547496260057No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAC249980.14545484167719358No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG233650.1359529712692067No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC228420.13290981252862052No Hit
TCAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGC227020.13209520024624566No Hit
CCTGAGCTCTACATCCTTTCCCTTCTCTGTGACCTTGTTACTGGGGGACT225440.13117585209899402No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG223770.13020413601930397No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT180460.10500352319812126No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG176260.10255968635099666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG3843900.082.2827451
AGTCTCT1106450.079.7077561
GTCTCTC1230100.071.669322
TCTCTCA1270350.069.3870243
CTGATGG4967750.063.7067682
TCAGCTG1395650.063.076397
CTCTCAG1403100.062.8359574
CAGCTGG1406700.062.5895048
TGATGGC5143300.061.5213133
GATGGCT5155000.061.3728944
TGGCTCA5199100.060.8279276
ATGGCTC5202000.060.814065
AGCTGGT1452200.060.6187259
GGCTCAA5304150.059.67077
CTCAGCT1484850.059.3789566
GCTCAAA5342900.059.2438748
CTCAAAC5383550.058.787719
TCTCAGC1512000.058.3692325
GTACTGC925050.052.04860384
ATACTGC924900.051.90261584
Produced by FastQC (version 0.11.5)