FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17291528
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC247560814.316883967686373No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC11635436.728977335027881No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC7147254.133382544330379No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3442751.991003918219373No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT1025900.5932963240726904No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT940240.543757613555031No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA755140.4367109719858187No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA712200.4118780017590117No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC686390.3969516169999551No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT455470.26340644967870974No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT446220.2580570091897026No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA438240.2534420324218889No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT418570.24206651951175165No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG378220.2187313926218666No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG372640.21550437879174125No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC329440.1905210459133513No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA326360.18873982680998463No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC314130.18166699900668118No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG299730.17333922138055122No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAG290090.16776423691416978No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG279570.16168033270396925No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC253200.14643008992611875No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAC250430.14482814936887012No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG234900.13584687252624522No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC228470.13212828848902192No Hit
CCTGAGCTCTACATCCTTTCCCTTCTCTGTGACCTTGTTACTGGGGGACT228110.13192009404836866No Hit
TCAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGC227490.1315615369561325No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG222850.1288781419432684No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT183860.10632952738474008No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG178980.10350733607810715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG3862800.082.258471
AGTCTCT1121900.079.75091
GTCTCTC1251400.071.45352
TCTCTCA1287300.069.475473
CTGATGG4988550.063.751132
CTCTCAG1414550.063.217654
TCAGCTG1415650.063.06327
CAGCTGG1426850.062.5451858
TGATGGC5165350.061.5468983
GATGGCT5171650.061.467534
ATGGCTC5220800.060.907545
TGGCTCA5219750.060.900766
AGCTGGT1473550.060.5628179
GGCTCAA5321100.059.7685627
CTCAGCT1506900.059.337466
GCTCAAA5360200.059.333098
CTCAAAC5402200.058.879419
TCTCAGC1528150.058.578795
ATACTGC923550.052.20420584
GTACTGC927750.051.78676684
Produced by FastQC (version 0.11.5)