FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39398693
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCA477750.12126036769798429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTGG144500.017.8627471
TGCGGGT100350.017.8486881
ATGGGAG107900.017.7768691
TGAGCCG118400.017.7629741
GGGACCT98550.017.677451
TGGCCAG124500.017.3746191
TGGGCGT66700.017.3624441
GGGAATG82150.017.3352661
CGAGCAC125800.017.3302151
TATGCCC156250.017.2619171
ACGAGCC99800.017.2556271
CCGGGAT90400.017.246691
AGATTGC154300.017.2143361
GACTGCG112400.017.1274451
TGACGGC69950.017.0847381
CGGACTG71100.017.0475181
CGCTGGA120950.017.0246661
GGCGACT78900.017.0226481
TTGACTT223050.016.9883231
ATGCGAT90100.016.9822331
Produced by FastQC (version 0.11.5)