FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19215535
Sequences flagged as poor quality0
Sequence length90
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC228351011.883666002533886No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC11486465.977694610116242No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC6898273.5899442820613636No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3578031.8620506793071336No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG2291891.192727654993733No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT1022580.5321631690192337No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC883110.4595812710913331No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT866160.45076028328120976No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT701510.3650744046418692No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC582410.3030933044539223No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG540390.2812255812809792No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT444360.23125039193548344No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA400710.20853439677843993No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA362080.1884308711675215No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGA352910.1836586907416317No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG330280.17188176129366162No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA320840.16696906955752208No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG288250.15000883399811663No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC287230.14947801349272866No Hit
TCAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGC249200.1296867352379208No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA226600.1179254181577562No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACC225180.11718643274829454No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA201780.10500878585998256No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG196280.10214651842896906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG3660100.081.8091
AGTCTCT1290100.079.8066561
GTCTCTC1441100.071.394442
TCTCTCA1509400.068.1575853
TCAGCTG1640300.062.699787
CTGATGG4791650.062.520412
CTCTCAG1659700.062.0412334
CAGCTGG1660100.061.9562158
TCTCAGC1683100.061.221585
AGCTGGT1683700.061.0770159
TGATGGC4934000.060.689853
GATGGCT4942400.060.5822034
ATGGCTC4999600.059.855355
TGGCTCA5006050.059.7653586
GGCTCAA5042550.059.380787
GCTCAAA5073650.059.0193378
CTCAGCT1753350.058.82346
CTCAAAC5106950.058.6353879
TGGTAAC364400.053.24719684
ATACTGC922200.051.76464584
Produced by FastQC (version 0.11.5)