FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17195965
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG53400.018.0014421
GGGAATG47800.017.7666261
GGCGACT49900.017.6804471
TAGCCGG68600.017.6727311
TGGCTGG38150.017.6197571
TGACGGC41850.017.5677871
ACTGAAC46700.017.2854941
ATGCGAT47500.017.2470761
CAATGGA53300.017.241688
GCGCGAT32300.017.1566471
CCGGAGG14750.017.0842044
ACGAGGA36050.017.036811
CGCGAAA48300.017.01829110
GTAACGG44850.017.0119210
TTGGAAC48100.016.9487511
CATGGCG34950.016.8861241
CTGAAAC50750.016.8718571
CGCGCCA37400.016.871329
CCGGGAT28700.016.8689731
GGGTTGC45600.016.846961
Produced by FastQC (version 0.11.5)