FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17421048
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTGG39800.017.99551
GGGAATG50800.017.9779851
ACTGATG57600.017.8179951
TGACGGC39400.017.6450371
ATGCGAT47800.017.6414241
GGCGACT49250.017.4876251
TGCGGGT32450.017.3859461
TTGAACG44050.017.3492761
TAGCCGG70450.017.294191
ACGAGGA39050.017.2652471
ACTGAAC48200.017.2044751
ACGAGCC34200.017.1982861
CGCGCCA38650.017.1794479
GCCGGAG20850.017.1697923
GCGCGAT32250.017.0905421
CAATGGA57350.017.0876878
GACCTGG33150.016.9282971
TTGGAAC53650.016.8924431
CGGACTG27600.016.8892691
CGTGAGA33000.016.87847110
Produced by FastQC (version 0.11.5)