FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18045760
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG50800.019.080811
GACCTGG69200.018.7197671
TGGATGG45000.018.66385310
GCGCGAT53050.018.5920931
GCGGGTG23500.018.2175432
TGAGAGG68350.018.1768991
GTAACTG86350.018.0369471
CGGACTG37050.018.0353681
CCTTACG75900.017.9766731
CGCTGGA42400.017.9537641
ATGCGAT61650.017.9456331
GGGTGAG36600.017.9281982
GGAACGG40400.017.86859310
AAGAAGG29350.017.8505547
TGCGGGT36850.017.7803611
TGACGGA39200.017.77793110
GTGCAGC56900.017.756031
TTGTAGG43700.017.6256851
GGGACCT30850.017.606841
ACGAGCC44600.017.606441
Produced by FastQC (version 0.11.5)