FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49072852
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACC508490.10361941058571447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT151400.018.4666421
TGACGGC115350.018.0809171
CATGGCG155400.018.0685541
TGGCTGG176350.017.9640661
GCGCGAT98750.017.8687231
CTCGAGG119550.017.8640671
GACCTGG154200.017.716151
ACAGCCG198800.017.621051
CGGACTG107500.017.5029951
ATGGGAG146350.017.4224471
GGGACCT99400.017.3996541
TACGGGC127400.017.1951791
ATTGGTG191250.017.0823171
TGAGCCG129150.017.0086561
ACGGAGA132550.016.994981
TGGGCGT90750.016.985881
CCACCTA319550.016.966511
TAGTTGG140850.016.9664651
CTGATAG164950.016.949681
CCGGGAT85600.016.8976611
Produced by FastQC (version 0.11.5)