FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48456916
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACC505180.1042534361864878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT153950.018.389951
CATGGCG152900.018.0451531
TGGCTGG174200.018.0037611
TGACGGC119800.017.9788131
CTCGAGG113700.017.9491351
GACCTGG147950.017.8712961
GCGCGAT96900.017.7676891
CGGACTG101850.017.5426121
ACAGCCG189000.017.334981
ATGGGAG147950.017.2897851
TAGTTGG132300.017.240461
TGCGGGT96800.017.165961
GGGACCT100050.017.1082951
TGAGCCG127500.017.0970381
ATTGGTG189950.017.0534171
TACGGGC128750.017.039831
CGGACGT130200.017.0344681
TGGGCGT91000.017.0068051
ACTGAAC128600.016.8963411
CCGGGAT87600.016.867481
Produced by FastQC (version 0.11.5)