FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17195965
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC245415414.271685247091396No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC11557586.721099978977627No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC7096674.126939081348445No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3425211.9918684412302539No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT1018720.5924180469080973No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT937430.5451453291513445No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA747740.43483456729529285No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA703450.4090785251074889No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC680640.39581378538511797No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT449020.261119396323498No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT445240.2589212062248324No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA432600.25157064462506173No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT414370.2409693204190634No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG371330.2159401929464267No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG366680.2132360702060047No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA330290.19207412901805743No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC323660.18821857336881065No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC308660.17949559678680435No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG298140.17337788254395725No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAG291600.16957466475420252No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG276670.16089239539624556No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC248980.14478978062586195No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAC247980.14420824885372818No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG234570.13640990778941456No Hit
TCAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGC228460.13285674866167732No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC228070.13262995127054517No Hit
CCTGAGCTCTACATCCTTTCCCTTCTCTGTGACCTTGTTACTGGGGGACT227030.13202515822752606No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG219990.12793117455170444No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT180940.10522235884988136No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG177500.10322188955374123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG3819200.082.337431
AGTCTCT1127050.079.6459351
GTCTCTC1251300.071.729252
TCTCTCA1291600.069.476443
CTGATGG4949650.063.5846372
TCAGCTG1417900.063.190937
CTCTCAG1420500.063.1902964
CAGCTGG1433300.062.5266238
TGATGGC5133150.061.307193
GATGGCT5147550.061.132584
ATGGCTC5194500.060.572365
TGGCTCA5198700.060.5160376
AGCTGGT1480300.060.4974259
GGCTCAA5295150.059.442647
CTCAGCT1510400.059.427496
GCTCAAA5333600.059.001528
TCTCAGC1531200.058.6920285
CTCAAAC5375800.058.5377779
GTACTGC924650.051.84029484
ATACTGC919700.051.68535684
Produced by FastQC (version 0.11.5)