FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39860694
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCA476550.11955386426538385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG110700.017.9333631
TGGCTGG142300.017.8516221
GGGACCT95100.017.7622491
TGAGCCG111900.017.7499851
CGAGCAC126050.017.5902371
TGCGGGT105600.017.5303781
TGGGCGT69200.017.4297051
GACTGCG114550.017.4179331
CCGGGAT84450.017.3850211
CGGACTG70450.017.2766041
GGGAATG85200.017.185031
GACCTGG90750.017.1809941
AGATTGC157600.017.1783261
TATGCCC163350.017.1491571
TGACGGC65800.017.1447851
TACGGGC134900.017.1331331
TGGCCAG132100.017.1328871
TAGTTGG96650.017.0738281
GGGAGAT74100.017.0194571
GGCGACT78150.017.007671
Produced by FastQC (version 0.11.5)