FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005493216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005493216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16624096
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTGG59450.018.2394621
TATGCCC52750.018.2236581
ATGGGAG54500.018.1891231
GTAACTG72450.018.100851
AGATTGC92000.017.8968521
CGAGCAC68350.017.884111
CTAATGG66050.017.825361
CGGACTG36550.017.6798971
GTGCTGC28550.017.6527639
TCTTGAG46400.017.6072838
CGGCTTG35250.017.5881799
TAGTTGG51250.017.566421
GGGACCT40550.017.5393451
ATGGAGA30100.017.4413914
GACCTGG56500.017.4036251
ATGCGAT50700.017.4018441
GGAAAGC42700.017.3824371
AGACTGG51750.017.3328658
AGGACGG19050.017.3224210
ATTGGCG25300.017.3117879
Produced by FastQC (version 0.11.5)