FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29373371
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG465630.15852113126545808No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT436320.14854270556825092No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG395620.1346866180255579No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG318990.10859836278239907No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT311430.10602460303245412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT45650.040.788216
CCGCGTT55800.033.3688557
AGACCGC59800.031.2162554
CGCGTTC65400.028.4706758
GCGTTCT68550.027.370279
GACCGCG68350.027.1028845
CGTACGG9950.026.2560313
CAGACCG73850.026.1778133
CGATTAG41650.022.4667724
GTTCGAT47000.021.134371
GCGTACG12650.020.6519742
TCGATTA43150.020.5849693
CGGTTCG34400.018.502733
GTCGGTT39000.017.6703131
TTCGCAC56700.017.5924228
GAACGAT55500.017.28812244-45
TTCGATT58150.017.2354322
TACGGAG61000.017.05311634-35
AATACGG61850.016.81875632-33
TTCGCGC31150.016.468547
Produced by FastQC (version 0.11.5)