FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29373371
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT412610.14047076857470667No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA397310.13526196908077046No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCG360200.12262807697488994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC154100.026.3921431
TTGAACG58000.023.5043377
ACGTGCG196800.020.8296176
AACACGT205550.020.1089323
TGCGCGA209250.019.9531929
GGTCGAC45200.019.8667551
CCGACCG139750.019.7477877
TGGCGCG134700.019.3638633
GGCGCGT137650.018.8071294
TAACACG219100.018.713532
TCTAACA222300.018.5937542
GGCGATC38400.018.558692
GTGCGCG232750.017.9386148
ACACGTG244300.017.0132314
CGTGCGC246750.016.766997
GAACGCT85450.015.8424279
CTAACAC284800.015.51481151
TGAACGC91200.015.2602788
CCCCGAC182600.015.1137065
CACGTGC285050.014.7974725
Produced by FastQC (version 0.11.5)