FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30470653
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT376400.12352869497086261No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT323430.10614475508614798No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA313860.10300402817097487No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA311060.102085111205198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT82000.038.1265531
GGCGATC86600.035.5515372
TGGCGCG226950.027.3409883
GGTGGCG259000.024.325361
GCGATCT134700.023.879343
GGCGCGT262400.023.5514564
CGATCTG137550.023.0685424
GTGGCGC281750.022.2424242
GCGCGTG317350.019.6528175
CGCGTGC320250.019.2968646
GGTCGAC54900.019.1264971
GCGTGCC350850.017.6680097
TTGAACG61500.017.0693027
CGGTGGC226750.017.0566061
TGCGACA198700.016.351476
GCGACAT203250.016.2191287
GCCACGA179200.015.51932618-19
CGTGCCT405100.015.3016278
GCTCATC266150.014.9560236
CTGCGAC227300.014.5448225
Produced by FastQC (version 0.11.5)