FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26715842
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA684250.2561214428502759No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG275430.10309613299854072No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA274360.10269562157165027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC41750.028.7871782
TGCGACA103400.027.0119576
GCGACAT103850.026.8947097
GGGCGAT48900.026.0366151
TGGCGCG127350.025.626663
CTGCGAC112400.025.0603775
GGCGCGT135500.024.2587534
GCTGCGA116400.023.9544854
TTCCGCA47800.021.4664423
GGTGGCG155450.021.1176641
GTGGCGC158000.020.745652
CGGAAAC148600.020.236881
GGCTGCG150250.018.8753093
CGATCTG66300.018.6277984
CGCAGGT57200.018.020346
GGTCGAC27750.017.4620721
GCGATCT75550.017.291493
GCGTGCC196800.017.2348752
CTTCCGC59700.017.1875692
TCCGCAG61050.017.1174354
Produced by FastQC (version 0.11.5)