FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26450536
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC53100.036.6780932
GGGCGAT56500.034.9757651
TGGCGCG155150.025.9937173
GGTCGAC59300.024.8330081
GGCGCGT173200.023.2837734
CGATCTG87500.023.1257134
GCGATCT94000.021.1232593
GGTGGCG201250.020.5355191
TTGAACG59550.019.9411877
GCGCGTG211350.019.1932455
GTGGCGC216450.019.1812
CGCGTGC215100.018.7261376
GCGTGCC235450.017.1479827
CGACCCG91200.017.0833324
CGTGCGG95200.016.0662089
GAACGCT73100.015.9849829
CGGTGGC174700.015.9612721
ACCCGTG97200.015.7355666
CCGTGCG104750.015.2816518
TGCGACA100500.015.1243496
Produced by FastQC (version 0.11.5)