FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28531829
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT936630.3282754848979363No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG608440.21324956069237624No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT550130.19281273555929415No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT464960.16296186269727048No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA378030.13249413488353656No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT350750.12293288313202774No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA320780.11242882466455269No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT307650.10782694653048705No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG303220.10627429457817092No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC302280.10594483795623476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG35500.047.097553
GCGTACG37100.045.322452
GGCGTAC48400.035.1474461
GTACGGA55750.030.1607694
TGGAGTC378550.025.546922
GAGTCTT384950.025.0975114
TACGGAA68050.024.7092295
CTCGCTA72250.024.4658971
AGTCTTG400000.024.1888435
GATCGTT67050.023.5192415
GGAGTCT417200.023.385153
TCGCTAT75400.023.1825122
TGATCGT68900.022.4741064
TTGGAAG454550.021.6830929
CGCTATG79200.021.4105113
TCTTGGA508450.019.6086737
GTCTTGG506100.019.5214026
CTTGGAA540400.019.258038
GTCGTAA27250.018.8265686-87
ACCGCGT30300.018.8114666
Produced by FastQC (version 0.11.5)