FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28531829
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT307850.10789704368409049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC61050.041.241482
GGGCGAT65150.038.647911
TGGCGCG200900.026.9540984
CGATCTG99550.026.0526124
CGGTGGC239700.025.6862981
GCGATCT104100.024.7337273
GGTGGCG241350.024.5253262
GGCGCGT221050.023.9598165
GTGGCGC257050.022.2140563
GCGTGCC304700.018.6935062
TGCGACA137000.018.0640986
CGCGTGC319400.017.9232481
GCGCGTG299350.017.8514046
GCGACAT140000.017.7448067
CGTGCCT359800.016.0023023
CTGCGAC159400.015.7342025
GCTGCGA162550.015.4585444
GGTCGAC38050.015.232451
CGCAGGG188600.013.8795221
TGTAGTC431950.013.4378329
Produced by FastQC (version 0.11.5)