FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28494770
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC53550.034.2420462
GGGCGAT63600.030.3264351
TGGCGCG166700.024.2507043
GCCGTAT54200.022.96110346-47
GGCGCGT177800.022.761824
CGTATCA59500.020.87594848-49
CGATCTG94500.019.9050144
GCGCGTG205850.019.7523945
CGCGTGC209000.019.3183256
GCGATCT100700.019.2469123
TTGAACG72400.018.5670937
CGGTGGC206950.018.4332791
GGTGGCG232700.018.39421
TCGCCGT67850.018.37674744-45
GGTCGAC41450.018.2232191
GCGTGCC229950.017.640897
GTGGCGC245400.017.4414312
CGCCGTA57050.016.90172846-47
GCGACAT112250.016.2918517
GAACGCT78550.016.2062159
Produced by FastQC (version 0.11.5)