FastQCFastQC Report
Sat 11 May 2024
EGAF00005494025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25154723
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC303640.12070894201458707TruSeq Adapter, Index 9 (100% over 50bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA270340.10747087137473149No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT256360.10191326694394527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)