FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25154723
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC303640.12070894201458707TruSeq Adapter, Index 9 (100% over 50bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA270340.10747087137473149No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT256360.10191326694394527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT68800.042.705696
CCGCGTT75250.039.04527
AGACCGC83650.035.739434
CAGACCG87700.034.5790673
GACCGCG91400.032.3538065
GCGTTCT92100.032.108039
GTCGGTT72100.030.8780981
CGCGTTC97600.030.2985448
CGGTTCG85600.028.2861213
TATGCCG57450.027.43036546-47
GGTTCGG73050.027.3272084
TCGTATG62100.027.13440142-43
GTTCGGT73800.026.7559575
TTCGGTC81050.024.5383056
CGTACGG9800.024.3659363
ATGCCGT69450.024.22855446-47
TCGGTCC85650.023.3312637
GTTCGAT30250.022.9003721
TCGGTTC94450.022.298512
TACGGAG84750.021.92681534-35
Produced by FastQC (version 0.11.5)