FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25154723
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG320600.12745121462875977Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT60650.026.54107346-47
CGTATCA63000.025.8148548-49
GGTCGAC66100.025.3642541
GGCGATC38650.025.0671252
CCGTATC63550.024.47063848-49
TTGAACG89800.023.901287
ACGTGCG86450.021.3116998
CGCCGTA74450.021.01552846-47
TGGTCGC76900.019.88291242-43
GAACGCT107600.019.858859
GGGCGAT53950.019.6318971
GTCTAAC99350.019.6003951
GGCGCGT110100.019.4946864
TGAACGC113150.019.3462898
GTGGTCG84900.018.90443840-41
TAACACG101500.018.3861854
TGGCGCG117500.018.1883873
AACACGT105200.018.0101055
TCGCCGT92200.017.99970444-45
ACCCGTG94750.017.8915546
Produced by FastQC (version 0.11.5)