FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34891186
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA963060.2760181324876718No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT643690.18448498712540182No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG402750.11543029807012006No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG350480.10044943728768636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT163650.058.688726
GACCGCG173950.055.4593775
AGACCGC175850.054.8331454
CCGCGTT178900.053.792117
CGCGTTC181800.052.9079138
CAGACCG197100.049.258793
GCGTTCT206800.046.5348559
TCAGACC320500.031.3600752
CTCAGAC361750.029.7972451
TACGGAG198450.026.7476734-35
AATACGG200950.026.56854632-33
ATACGGA196900.025.60729832-33
CAATACG197050.025.58780730-31
GAACGAT195850.025.56268744-45
AACGATC207250.025.52026246-47
GCAACGC205450.024.53006680-81
CGATCAT217750.024.40964148-49
CAACGCC217200.024.29650182-83
AGAACGA223600.023.69666744-45
TGACGGC232100.022.82884462-63
Produced by FastQC (version 0.11.5)