FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36394282
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1372940.37724057861616833No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC737660.20268568562501107No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG568010.15607122019882133No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA485540.13341106715609885No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA466040.12805308262435292No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT421030.11568575525133316No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC392840.1079400329974912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG20850.032.7536433
ACCGCGT71200.030.0663246
AGACCGC82250.026.534444
CGCGTTC80800.026.435348
CCGCGTT84000.025.654317
GCGTACG26850.025.4343912
GACCGCG94850.022.6195985
GCGTTCT98500.021.781419
CAGACCG106800.020.9268673
TGGAGTC730900.020.5621452
GAGTCTT735450.020.4544124
GGAGTCT752950.020.2517433
AGTCTTG776350.019.5648375
GTCTTGG818100.018.7578536
CTTGGAA833700.018.0083188
GTACGGA40050.017.7669724
TTGGAAG848600.017.6921239
TCGACCC70550.017.667423
TCTTGGA882600.017.4193047
AATACGG86600.015.84031132-33
Produced by FastQC (version 0.11.5)