FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28429282
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT934050.3285520893563193No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT911720.32069751181194095No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG837940.29474539666531147No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA607530.21369867870739753No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC587780.2067516161681466No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC547730.19266402858855175No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG388380.13661266577186154No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG365950.12872291322728446No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA344440.12115677068453576No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT330060.11609860565595712No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC322340.1133830956406145No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT315040.11081532062610656No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATG306300.10774102560873679No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA306060.10765660560825982No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT296250.10420593808876356No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC291480.10252809057928372No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT285860.10055125556811459No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT285810.10053366806801524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAC292550.033.4398465
CGTACGG17250.032.3899463
TTCGTCC196900.030.8742282
CTTCGTC204650.030.1134471
TCGTAAC66550.030.03096688-89
GTCGTAA66850.029.82475986-87
AGTCGTA68600.029.79487486-87
GCGTACG18900.029.5622482
TTGACTG343250.028.9569327
GAGTCTT328050.027.876754
CGTCCTG226400.027.4840934
TGGAGTC334650.027.2984262
TGATCGT70800.027.249694
CGTAACA73150.027.19083688-89
GAGTTTG365000.027.1873023
AGTCTTG347150.026.5122785
GATCGTT73200.026.1324625
GACTGGG376000.026.0686999
GGAGTCT359700.025.8088843
AAGTCGT79200.025.74664984-85
Produced by FastQC (version 0.11.5)