FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28429282
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG852650.29991963919454595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT57850.031.2817731
GGCGATC57400.031.1115782
CGGAAAC327300.029.7783811
TTCCGCA95950.029.5015683
AACCTTG383950.026.19465
TGGCGCG156700.026.1266882
GGAAACC386650.025.6851482
ACCTTGT400100.024.9236266
CTTCCGC114900.024.8841342
GAAACCT414200.024.4352683
CCTTGTT407750.024.188177
CTTGTTA405050.024.137968
CGCAGGT122000.023.5091886
TGCGACA121550.023.3227586
GCGACAT123150.023.2896547
AAACCTT440450.023.0830294
TCCGCAG124350.022.8364264
GGCGCGT185450.022.2298563
CGATCTG87400.021.0267544
CTGCGAC138950.020.7780865
Produced by FastQC (version 0.11.5)