FastQCFastQC Report
Wed 1 May 2024
EGAF00005494035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28966823
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA619650.21391714238043988No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT582030.20092987070069782TruSeq Adapter, Index 13 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT444160.15333404011893192No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC409460.14135481823464038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)