FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28966823
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA619650.21391714238043988No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT582030.20092987070069782TruSeq Adapter, Index 13 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT444160.15333404011893192No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC409460.14135481823464038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT142000.044.6228376
GACCGCG151450.041.901245
CGCGTTC152650.041.5407338
CCGCGTT154500.041.104817
AGACCGC157950.040.2971954
CAGACCG163000.039.6024063
GCGTTCT171050.037.0721639
TCGTATG111500.034.8046244-45
CGTACGG12050.032.323363
ATGCCGT129100.030.05976148-49
CGGTTCG75250.029.6675073
GTCGGTT82750.029.1751171
TATGCCG105150.028.52684648-49
GTTCGGT65750.028.102415
AATACGG159150.026.5030632-33
GCCGTCT146400.026.31294650-51
GGTTCGG71250.026.266434
GTATGCC150650.026.0278146-47
TACGGAG163800.025.80868134-35
TCTCGTA139950.025.4722642-43
Produced by FastQC (version 0.11.5)