FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27867112
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC49950.026.8129832
GTCTAAC87350.025.88231
ACGTGCG90550.024.1224488
GGGCGAT59850.023.7282581
TAACACG99450.022.1074754
GGCGCGT162700.021.3641914
TGGCGCG167300.020.524343
AACACGT110500.020.45525
TGCGCGA110700.020.289349
CGCGTGC201200.017.4647676
GGTGGCG202800.017.3777981
TTACAGC193300.017.0759352
ACACGTG132700.017.0689376
GTTACAG212350.016.4620671
TACAGCC206450.016.1721323
GTGCGCG136800.016.105868
GGTCGAC39250.016.0942521
GCGCGTG223150.015.9170935
GCGACAT95750.015.8201627
TGCGACA94550.015.8200576
Produced by FastQC (version 0.11.5)