FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31410588
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1538270.48972976882826896No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT940700.2994850016815986TruSeq Adapter, Index 13 (97% over 40bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG885080.28177759677723957No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC542070.17257556592063797No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC483730.15400221097421035No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA421000.13403123812900286No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT402750.1282210953835057No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC380150.12102606929867087No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA354450.11284411485706666No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT353430.11251938359129095No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT329530.10491048432458507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG184700.037.6746544-45
ATGCCGT206100.033.5209548-49
TCTCGTA199850.032.08731542-43
GTATGCC223050.031.27155946-47
AATCTCG206400.031.1495340-41
GCCGTCT226450.030.37233550-51
TATGCCG177300.028.4046148-49
ACCGCGT43150.025.7405136
TGGAGTC634000.025.6716562
GGAGTCT649550.025.2261923
CGTCTTC274700.025.08075152-53
GAGTCTT649550.024.9615044
AGTCTTG682650.023.9954575
CGTATGC216350.023.49712646-47
CGCGTTC47600.023.4338198
ATCTCGT211750.022.92039142-43
CCGCGTT50050.022.1918727
CGTACGG12100.022.1027473
GTCTTGG753550.021.9393396
CTTGGAA742350.021.9058848
Produced by FastQC (version 0.11.5)