FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31410588
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG966950.30784205631553285Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA476850.15181186675015446No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT372510.11859376844521345Illumina Single End PCR Primer 1 (100% over 50bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT342020.10888685051040752No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG323170.10288568937327758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT85100.041.63471
CGTATCA181200.037.6177748-49
GCCGTAT184300.036.8690846-47
GGCGATC99950.035.1146052
TCGCCGT216800.031.42970544-45
GTGGTCG235100.029.15540540-41
CGCCGTA175400.028.72326546-47
CCGTATC185900.027.24140248-49
CGGTGGT261400.026.32193238-39
GGTCGCC262900.026.01793142-43
CTCGGTG272250.025.40373436-37
ATCTCGG285800.024.82233434-35
GTAGATC286950.024.61493930-31
TGGTCGC212450.023.59128642-43
TGGCGCG219450.023.0481853
GCGATCT158000.022.8443723
GGTGGCG245600.022.4710831
CGATCTG164150.021.6096524
GAGTGTA334850.021.30631826-27
TCTCGGT238700.021.1065836-37
Produced by FastQC (version 0.11.5)