FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31509115
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT71250.026.43326246-47
CGTATCA73950.025.46815548-49
GGCGCGT158450.022.3936544
TGGCGCG163100.022.0478423
GGCGATC53400.021.1719022
GGTCGCC94100.020.29218942-43
GGTGGCG194650.019.0115221
TCGCCGT102650.018.83336644-45
CGCCGTA58050.018.73810846-47
GTGGTCG105200.018.24151240-41
GAGATAC184850.018.0659183
CGCGTGC196400.018.01826
GCGCGTG202000.017.5657165
GATACCA191350.017.1780645
GGGCGAT77300.017.0843771
TCACGAA133550.016.62770314-15
GGTCGAC46700.016.2755951
GGAGATA221950.016.1376532
GTGGCGC229700.015.8620222
AGATACC214450.015.5270374
Produced by FastQC (version 0.11.5)