FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30287125
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1057900.34929033376393437TruSeq Adapter, Index 7 (100% over 50bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA585410.1932867513836325No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT455440.15037412762023467No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG357930.1181789291654457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT125450.044.1867186
GACCGCG130450.042.420275
CCGCGTT132900.041.7097367
AGACCGC135200.041.035314
CGCGTTC136800.040.5206388
CAGACCG141200.039.6283
TCGTATG181550.039.57230842-43
ATGCCGT198700.035.8818146-47
GCGTTCT160850.034.668789
TCTCGTA188050.034.0493540-41
TATGCCG176550.033.85928344-45
GCCGTCT211850.033.29580348-49
GTATGCC221550.032.72783744-45
CGTCTTC244950.028.83533950-51
CGTATGC209000.028.64763842-43
TGCCGTC216300.027.17566746-47
CATCTCG240650.026.77479638-39
CCGTCTT221400.026.44239848-49
ATCTCGT199500.026.32134438-39
GAACGAT149550.023.646744-45
Produced by FastQC (version 0.11.5)