FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30287125
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1209160.3992323470781727Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT178300.039.82744246-47
CGTATCA183500.038.63416748-49
TCGCCGT199100.035.79788244-45
CGCCGTA168450.034.93758444-45
GGTCGCC214400.033.91899542-43
GTGGTCG222150.032.5754840-41
CCGTATC184000.032.0495146-47
TGGTCGC206100.029.0969940-41
CGGTGGT249950.029.0758938-39
CTCGGTG259000.028.24331336-37
GGGCGAT54950.028.18271
GTCGCCG225250.026.90781642-43
TCTCGGT231050.026.20162634-35
TCGGTGG234200.025.87965636-37
ATCTCGG296900.025.1179334-35
GTAGATC305650.023.94035330-31
GGCGATC62200.023.9044512
GGCGCGT151150.022.4714261
GTATCAT266250.022.30937448-49
TGGCGCG155050.021.813853
Produced by FastQC (version 0.11.5)